A few plasmids (pGL1124, pGL1224 and pGL1217) ended up made to enable the replacement of one allele of CYC9 Lister 427 cells. Trypanosomes have been transfected with each plasmid independently, and afterwards subjected to some 2nd transfection with one of many other plasmids, with another resistance gene, with all pairwise https://dbibb13310.bloggip.com/30157757/details-fiction-and-2r-2-pcca-hydrochloride